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  What is genetically modified food?
Genetically modified foods (abbr. GMF) refer to the transition of some genes from one creature to other species by using transgenic technology, so as to transform the latter’s genetic material. The change in shape, consumer behavior and nutrition conforms to people’s needs.
The features of GMF
1.The genome in GMF itself and its raw material is artificially changed, which differs from traditional food. These changes include the change in growth rate, the increase of output and the removal of some genes that are unnecessary or harmful to humans. However, due to the limitation of science and technology, there might be a few unexpected results in the process of change and huge uncertainty of human’s adaptability. This is the fundamental reason that the food safety and stability of GMF are lower than the traditional one.
2.The development of GMF is a certain prospect and value. In many Third World countries, GMF not only improves the adaptability, nutrition and biological features of crops, but also improves the utilization rate of farmland. Meanwhile, GMF maintains the fertility of land and reduces the side effects of pesticide. In a word, GMF expands the boundary in the field of food. Moreover, it promotes the development of prospective new food marketing while satisfying the people’s needs of food, which pushes the development of economy.
 
  How to test GMF?
The testing methods of GMF develop rapidly. Many corresponding testing methods have derived from the different testing materials. ICAS gene laboratory uses suitable, efficient and accurate testing methods depending on different food types and processing methods, as well as different corresponding transgenic fragments in the GMF. The most common testing methods can be divided into nucleic acid test and molecular test.
1. Nucleic acid test
a. Qualitative PCR technology
In DNA extraction, the promoter and terminator’s exogenous gene that are mentioned above are equipped with primer, and then proper amplification is applied to the DNA of GMF which is to be tested through effective equipments. Determination is based on any DNA sequences and fragments with special length and sequence.
b. Quantitative PCR technology
Quantitative test is necessary since many countries have strict requirements on the percentage of transgene in the food. For test according to reference object, Quantitative polymerase chain reaction test is to analyze final product or process monitoring of PRC, and further assess the copy numbers of target gene in transgenic food sample. The common ways of testing GMF quantitative polymerase chain reaction are: semi quantitative reverse transcription polymerase chain reaction, real-time fluorescence quantitative polymerase chain reaction and quantitative multiplex polymerase chain reaction.
a. Blot
Whether the Southern blot for exogenous gene or Northern blot for RNA, the basic process to be tested is to transfer nucleic acid segment, which is to be tested, and further bind to a specific solid support. Then, use nucleic acid probe which is marked in advance to test the nucleic acid.
2. Transgenic protein test
a. Two-dimensional electrophoresis
Two-dimensional electrophoresis, one of the key technologies researched by team protein, is the key technology to separate protein. It can separate protein from two samples by high resolution and high sensitivity. The determination of this technology is to separate protein from two samples with physical or chemical principles.
b. Western hybridization test
This method separates protein molecule according to its size, and then add specific target protein antibody, to combine imprinting protein of purpose molecular. After adding specific enzyme to 1st antibody, the combination is marked as 2nd antibody. Finally, the performance of marked combination can be tested by 2nd antibody.
Service range of gene test
Service Project classification Testing range
Transgenic test ■ Qualitative test on transgenic ingredient in crops
■Qualitative test on transgenic ingredient in food and condiments
1. Common crops, such as soybean, maize, rice, wheat, potato, papaya, rape, etc.2. Ordinary food and condiments
Animal origin test ■ Animal origin test in food
■ Animal origin test in gelatin
1. Common crops, such as soybean, maize, rice, wheat, potato, papaya, rape, etc.2. Ordinary food and condiments
Allergen test ■ Food allergen-caused ingredient test Allergen such as peanut, soybean and wheat.

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ICAS gene laboratory professional inspection team provides food production and circulation enterprises, inspection institutions and research institutes with comprehensive, rapid, simple and convenient, and economical professional gene inspection for your product security!

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Website: icasiso.com.cn

WeChat Public ID: ICAS英格尔

Customer Service   (0086) 400-182-9001

 

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INTERNATIONAL CERTIFICATION AND ASSESSMENT SERVICE PTY LTD 

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